Journal: Journal of Molecular Biology

Article Title: Discriminative SKP2 Interactions with CDK-Cyclin Complexes Support a Cyclin A-Specific Role in p27KIP1 Degradation

doi: 10.1016/j.jmb.2020.166795

Figure Lengend Snippet: Defining the SKP2 binding site on cyclin A. (a) Surface plasmon resonance to measure the interaction between p27 and CDK2-cyclin A and CDK2-cyclin Amut5. GST-p27M (residues 1–106) was bound to a CM5 sensor-chip functionalized with anti-GST antibody and the CDK2-cyclin A complexes were flowed over at increasing concentrations. K d values were derived using Biacore S200 evaluation software. SPR sensorgrams are representative of two repeats using independently prepared protein samples. (b) Isothermal titration calorimetry (ITC) to measure the interaction between p27MS (residues 23–51) and CDK2-cyclin A complexes. K d values and reaction conditions are tabulated in . (c) Cyclin A mutants distinguish SKP2 and p27 binding sites on cyclin A and reveal their shared and divergent binding elements. ITC thermograms for the binding of p27MS (residues 23–51) and SKP1-SKP2N to cyclin A_W217K, cyclin A_I213D (within the cyclin A α1 MRAIL sequence), cyclin A_R293E (on cyclin α5). ITC thermograms for other mutants discussed in the text are included in Figure S3(A). ITC thermograms shown are of one of two replicates carried out using independently prepared proteins. Data analysis is described in .

Article Snippet: SPR analysis was carried out using a Biacore S200 (GE Healthcare).

Techniques: Binding Assay, SPR Assay, Derivative Assay, Software, Isothermal Titration Calorimetry, Sequencing

Journal: Cell

Article Title: In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies

doi: 10.1016/j.cell.2021.06.021

Figure Lengend Snippet:

Article Snippet: Results were analyzed using the Biacore S200 Evaluation software (Cytiva).

Techniques: Recombinant, Staining, Random Hexamer Labeling, Luciferase, Cell Culture, Lysis, Multiplex Assay, Reporter Gene Assay, Binding Assay, Expressing, Transgenic Assay, Sequencing, Software